rabbit polyclonal antibody against hsp60  (Cell Signaling Technology Inc)

Journal: iScience

Article Title: TFAM expression in brown adipocytes confers obesity resistance by secreting extracellular vesicles that promote self-activation

doi: 10.1016/j.isci.2022.104889

Figure Lengend Snippet: Effects of hTFAM overexpression on white and brown adipose tissue phenotypes (A) Verification of hTFAM protein expression in epididymal (e)- and inguinal (i)-WAT and BAT. (B) The h- and m- TFAM protein levels were evaluated using western blot analysis. Pictures represent western blots for h- and m- TFAM in BAT. (C) The ratio between hTFAM and mTFAM protein expression in Tg and TgTg BAT. (D) The h- and m- TFAM protein levels were normalized by the levels of HSP60. The quantitative results are expressed as the mean percentage of the level in the Tg ± standard error (SE) (n = 3/group). Total protein extracts from e− and i-WAT and BAT probed with a polyclonal antiserum against h- and m- TFAM. (E) Photographs show representative conditions of e − and i-WAT, liver, and BAT from NCD-fed WT, Tg, and TgTg mice. (F) Effect of hTFAM overexpression on e-adipocyte size. Pictures are representative of each sample stained with hematoxylin and eosin and examined by optical microscopy. Scale bar represents 100 μm. (G) Frequency distribution of the e-adipocyte area in NCD-fed WT, Tg, and TgTg mice. ∗∗p < 0.01, versus Tg mice. Two-tailed unpaired Student’s t test in (C) and (D).

Article Snippet: Rabbit polyclonal anti HSP60 (D307) , Cell Signaling Technology , Cat# 4870; RRID: AB_2295614.

Techniques: Over Expression, Expressing, Western Blot, Staining, Microscopy, Two Tailed Test

Journal: iScience

Article Title: TFAM expression in brown adipocytes confers obesity resistance by secreting extracellular vesicles that promote self-activation

doi: 10.1016/j.isci.2022.104889

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti HSP60 (D307) , Cell Signaling Technology , Cat# 4870; RRID: AB_2295614.

Techniques: Staining, Recombinant, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Bicinchoninic Acid Protein Assay, Software, Membrane, Chromatography, Transmission Assay, Microscopy

Journal: Molecular cell

Article Title: Partitioning of MLX-Family Transcription Factors to Lipid Droplets Regulates Metabolic Gene Expression

doi: 10.1016/j.molcel.2020.01.014

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Cat# 4870S Rabbit polyclonal anti-IgG Cell Signaling Techn.

Techniques: Binding Assay, Recombinant, Transfection, Sequencing, Staining, Chromatin Immunoprecipitation, PCR Cloning, Bicinchoninic Acid Protein Assay, cDNA Synthesis, SYBR Green Assay, RNA Sequencing, Control, Cloning, Plasmid Preparation, Software